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ABSTRACT

  • 1Dr Tsakani Rasakanya,
  • 2Prof Elzbieta Osuch
  • 1Sefako Makgatho Health Sciences University
  • 2Sefako Makgatho Health Science University

Background

Endothelial dysfunction (ED) is defined as a transformation towards injurious processes which contributes to vasoconstriction, excessive thrombosis, and irregular vascular proliferation. Endothelin-1 (ET-1) has been shown to be responsible for maintaining arterial stiffness. In ED, where nitric oxide (NO) production is low and that of ET-1 is high, the balance is changed to elevate arterial stiffness. ED depreciates more rapidly in patients with type 2 diabetes mellitus, possibly due to the toxic consequences of hyperinsulinemia in the vascular wall.
Aim: To Investigate the effects of an eight-month treatment with metformin alone and in combination with glimepiride on arterial elasticity, endothelial dysfunction (ET-1) in black type 2 diabetes mellitus patients.

Method

The AtCor SphygmoCor® system (AtCor Medical, Inc., Sydney, Australia) was used to measure PWV and Aix. Endothelin-1 levels were measured using Multiplexing with Bio-Plex Pro™ human inflammation panel I assay. Type 2 diabetic treatment naïve participants were divided into two groups: metformin (M) (n = 10) and metformin glimepiride (MS) (n = 14). After treatment initiation study participants were followed up at 4 and 8 months.

Result

In the M and MS group, ET-1 levels significantly decreased at four months (7.54±4.51 pg/ml, 5.04±3.31 pg/ml) and at eight months (3.25±3.38 pg/ml, 5.09±3.01 pg/ml) when compared to baseline (24.10±11.31pg/ml) (P<0.05). In the M group, there was also a statistically significant decrease in ET-1 levels at eight months (3.25±3.4 pg/ml) when compared to four months (7.54±4.5 pg/ml) (P<0.05). PWV was directly correlated to ET-1 (r2=0.16073; P=0.0450). There was no correlation between Aix and ET-1 (r2=0.09838; P=0.2218).

Conclusion

Metformin alone and in combination with glimepiride significantly decreased ET-1 levels over the period of eight months treatment. There was positive correlation between PWV and ET-1 levels indicating possible improvement of arterial elasticity with longer-term T2DM treatment in black participants.
Author Image

PRESENTING AUTHOR

Dr. Tsakani Rasakanya,

Senior Lecturer, Sefako Makgatho Health Sciences University

Senior Lecturer and Research Coordinator
Department of Pharmacology and Therapeutics
Sefako Makgatho Health Sciences University, Ga-Rankuwa, South Africa

Professional qualification(s)
2023 PhD, Pharmacology, Sefako Makgatho Health Science University
2014 MSc, Pharmacology, University of Pretoria
2009 BSc (Hons) Pharmacology, University of Pretoria
2008 BSc (Medical Cell Biology and Human Physiology), University of the Witwatersrand

Registrations
• Health Professions Council of South Africa, Registered number MSS0005932
• South Africa Society of Basic and Clinical Pharmacology, Registration number 100-3203

Current Positions and accolades
Present Senior Lecturer and Research coordinator, Department of Pharmacology and Therapeutics, Sefako Makgatho Health Sciences University, South Africa (SMU)
2023 - Present Academic coordinator for Post-graduate studies and Research, Department of Pharmacology and Therapeutics, SMU
2014 – Present Academic coordinator for Nursing programme, Department of Pharmacology and Therapeutics, SMU
2023 – Present Member of the Postgraduate Examination Committee, Sefako Makgatho Health Science University

Awards and Recognitions
• First Place in the Young Pharmacologist award, Category “Best Podium Presentation in Clinical Pharmacology ”, 2023

ARTICLES IN PEER REVIEW JOURNALS
• T Rasakanya, E Osuch; Manuscript title: Effect of metformin on arterial elasticity and pro-inflammatory markers in black type 2 diabetes mellitus patients, Journal: Health SA Gesondheid, ISSN: 1025-9848, E-ISSN: 2071-9736
• Keetile, N.; Osuch, E.; Lentoor, A.G.; Rasakanya, T. Association of Circulating Levels of Inflammatory Cytokines and Chemotherapy-Associated Subjective Cognitive Impairment in a South African Cohort of Breast Cancer Patients. NeuroSci 2023, 4, 296-304. https://doi.org/10.3390/neurosci4040024
• Rasakanya TL, Osuch E. Arterial stiffness assessment in obese black South African patients. Cardiovasc J Afr. 2023 Feb 13;34:1-5
• E Osuch, TL Rasakanya. Pharmacology and therapeutic uses of antiplatelet drugs. South African General Practitioner 2020; 1(3):101-106
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